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How to judge gel filtration, target protein in zeolite molecular sieve chromatography

   Gel filtration is the separation of different substances by molecular size. Many times, it is used for desalting of protein solutions; for protein solutions whose molecular weight is determined, the elution time of the target protein can be estimated by comparing the molecular weights, if uncertain The nature of the protein can only be followed by the identification of each protein peak. How can we estimate the elution time of the target protein by comparing the molecular weights?

     Large molecular weight, can not enter the gel pores, the elution time is short, the small molecular weight, will enter the gel pores, the elution time is long, but this is relative, such as two large protein molecules, can not enter the inner pore , then they will elute at the same time, can not be separated, so the gel is suitable for the separation of proteins with different molecular weights.